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Terahertz Microfluidic Sensing Using a Parallel-plate Waveguide Sensor

Terahertz Microfluidic Sensing Using a Parallel-plate Waveguide Sensor

Published on: August 30, 2012

Design and fabrication of biosensing interface for waveguide-mode sensor.

Mutsuo Tanaka¹, Kyoko Yoshioka, Yoshiki Hirata

¹Biomedical Research Institute , Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.

Langmuir : the ACS Journal of Surfaces and Colloids

|September 26, 2013

Summary

A novel biosensing interface using triethoxysilane derivatives and anti-leptin antibody was developed for waveguide-mode sensors. This system effectively detects human leptin in serum at nanogram levels without labeling.

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Area of Science:

Biomedical Engineering
Materials Science
Analytical Chemistry

Background:

Development of sensitive and specific biosensing systems is crucial for early disease diagnosis.
Waveguide-mode sensors offer potential for label-free detection of biomarkers.
Immobilization of antibodies onto sensor surfaces is key for specific antigen recognition.

Purpose of the Study:

To fabricate a biosensing interface on a silica waveguide chip using triethoxysilane derivatives and anti-leptin antibody.
To optimize the interface for suppressing non-specific protein adsorption while maintaining antibody activity.
To evaluate the performance of the developed waveguide-mode sensor for detecting human leptin.

Main Methods:

Synthesis of triethoxysilane derivatives functionalized with succinimide ester for antibody immobilization and oligoethylene glycol for anti-fouling properties.
Modification of silica waveguide chips with these derivatives and anti-leptin antibody.
Characterization of non-specific adsorption using human serum and evaluation of antigen-antibody reaction response and stability.

Main Results:

The oligoethylene glycol moiety effectively suppressed non-specific protein adsorption from human serum.
The succinimide ester moiety enabled successful antibody immobilization, leading to antigen-antibody reaction detection.
The optimized biosensing interface achieved detection limits of 50 ng/mL in PBS and 100 ng/mL in human serum for human leptin.

Conclusions:

The fabricated biosensing interface demonstrates effective antibody immobilization and anti-fouling properties.
The waveguide-mode sensor system shows promise for label-free detection of biomarkers like leptin in complex biological samples.
The developed system has potential for detecting biomarkers at concentrations in the tens of nanograms per milliliter range.