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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
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Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...
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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

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Related Experiment Video

Updated: May 7, 2026

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy
08:49

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

Static hyperspectral fluorescence imaging of viscous materials based on a linear variable filter spectrometer.

Patrik J Murr1, Michael Schardt, Alexander W Koch

  • 1Institute for Measurement Systems and Sensor Technology, Technische Universität München, Theresienstrasse 90/N5, Munich 80333, Germany. patrik.murr@tum.de.

Sensors (Basel, Switzerland)
|September 26, 2013
PubMed
Summary

This study introduces a low-cost hyperspectral imaging setup for detecting impurities in viscous materials using fluorescence. The system achieves high-resolution imaging and can identify particles down to the nanometer range.

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Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
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Last Updated: May 7, 2026

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Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
07:34

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Area of Science:

  • Spectroscopy
  • Materials Science
  • Analytical Chemistry

Background:

  • Hyperspectral imaging offers detailed spectral information for material analysis.
  • Detecting impurities in viscous materials is crucial for quality control.
  • Existing methods may lack cost-effectiveness or resolution.

Purpose of the Study:

  • To develop and validate a low-cost hyperspectral measurement setup for fluorescence detection.
  • To enable the imaging and detection of fluorescent and non-fluorescent impurities in viscous materials.
  • To demonstrate the capability of identifying micro-scale surfaces and nano-scale particles.

Main Methods:

  • Utilized a narrow-band light-emitting diode (LED) at 370 nm for illumination.
  • Employed a linear variable filter (LVF) covering 400-700 nm with a complementary metal oxide semiconductor (CMOS) 2D sensor.
  • Performed static measurements on fluorescent viscous materials with non-fluorescent impurities.

Main Results:

  • Successfully acquired hyperspectral fluorescence images of viscous materials.
  • Demonstrated detection of both fluorescent and non-fluorescent impurities.
  • Achieved measurement surface resolution in the micrometer range and minimum particle size detection in the nanometer range.
  • Confirmed recording rates in the microsecond range.

Conclusions:

  • The presented low-cost hyperspectral setup is effective for impurity detection in viscous materials.
  • The system offers high sensitivity and resolution for micro/nano-scale analysis.
  • This technology has potential applications in quality control and material characterization.