Related Experiment Video
Updated: May 7, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
Class A β-lactamases as versatile scaffolds to create hybrid enzymes: applications from basic research to medicine
Céline Huynen1, Patrice Filée, André Matagne
1Laboratory of Enzymology and Protein Folding, Centre for Protein Engineering, Institute of Chemistry, University of Liege, (Sart-Tilman) 4000 Liege, Belgium.
Abstract:
Designing hybrid proteins is a major aspect of protein engineering and covers a very wide range of applications from basic research to medical applications. This review focuses on the use of class A β-lactamases as versatile scaffolds to design hybrid enzymes (referred to as β-lactamase hybrid proteins, BHPs) in which an exogenous peptide, protein or fragment thereof is inserted at various permissive positions. We discuss how BHPs can be specifically designed to create bifunctional proteins, to produce and to characterize proteins that are otherwise difficult to express, to determine the epitope of specific antibodies, to generate antibodies against nonimmunogenic epitopes, and to better understand the structure/function relationship of proteins.
More Related Videos
Related Concept Videos
Inhibitors of Gram-positive Cell Wall Synthesis
Combined Effects of Drugs: Synergism
Such synergistic combinations...
Production of Antibiotics
Production of Pharmaceuticals
Biosynthesis in Bacteria
Clinical Significance of Antibiotic Resistance

