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Updated: May 7, 2026
Establishment and Validation of a Rat Model of Pulmonary Arterial Hypertension Associated with Pulmonary Fibrosis
Published on: May 23, 2025
[Effect of pulchinenoside in regulating FLS SFRP2 expression of RA model rats]
Cheng-Gui Miao1, Jian-Ting Yang, Hua-Qi He
1Anhui Key Laboratory of Poultry Disease Monitoring, Anhui Science and Technology University, Bengbu 233100, China. fensheng1981@126.com
Objective:
To study the effect of pulchinenoside (PULC) in modulating SFRP2 expression in fibroblast-like synoviocytes (FLS) of rheumatoid arthritis (RA) model rats.
Method:
The effect of PULC in treating RA rats was evaluated by rat arthritis score and paw swelling score. The inhibitory effect of PULC on FLS proliferation was detected by MTT reagent. The effects of PULC gavage treatment in modulating gene expression of FLS SFRP2, critical gene beta-catenin of Wnt pathway and downstream effector genes C-myc of of Wnt pathway were detected by RT-PCR and Western blotting.
Result:
PULC had a significant effect in treating RA rats and that SFRP2 expression was down-regulated in FLS. After PULC gavage treatment, FLS SFRP2 expression was obviously up-regulated, whereas beta-catenin and C-myc gene expressions were significantly down-regulated.
Conclusion:
PULC can inhibit abnormal proliferation of synovial membrane by modulating Wnt pathway of RA rats.
Insights
Pulchinenoside (PULC) effectively treats rheumatoid arthritis (RA) in rats by modulating fibroblast-like synoviocytes (FLS). It up-regulates SFRP2 and down-regulates Wnt pathway genes, inhibiting abnormal synovial proliferation.
Area of Science:
- Rheumatology and Molecular Biology
- Investigating the molecular mechanisms of rheumatoid arthritis (RA) and potential therapeutic interventions.
Context:
- Rheumatoid arthritis (RA) is characterized by abnormal proliferation of fibroblast-like synoviocytes (FLS).
- The Wnt signaling pathway plays a critical role in the pathogenesis of RA.
- SFRP2 is implicated in the regulation of FLS behavior in RA.
Purpose:
- To investigate the therapeutic effect of pulchinenoside (PULC) on RA.
- To elucidate the impact of PULC on SFRP2 expression in FLS from RA model rats.
- To examine PULC's modulation of the Wnt pathway, including beta-catenin and C-myc.
Summary:
- Pulchinenoside (PULC) treatment significantly improved arthritis and paw swelling scores in RA rats.
- PULC treatment led to the up-regulation of SFRP2 expression in FLS.
- PULC significantly down-regulated the expression of beta-catenin and C-myc, key components of the Wnt pathway.
Impact:
- PULC demonstrates potential as a therapeutic agent for rheumatoid arthritis.
- Modulation of SFRP2 and the Wnt pathway by PULC offers a novel therapeutic strategy for RA.
- This study provides insights into the molecular mechanisms underlying PULC's anti-arthritic effects.