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Updated: May 7, 2026

Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes
Published on: May 19, 2019
[Relationship between heroin spongiform leucoencephalopathy and respiratory chain complex I deficiency]
Liang Zhou1, Minshi Lin, Jia Yin
1Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: zhouliang_1963@126.com.
Objective:
To investigate the relationship between heroin spongiform leucoencephalopathy and respiratory chain complex I deficiency.
Methods:
The activity of respiratory chain complex I in peripheral white blood cell mitochondria was compared between 36 cases of heroin spongiform leucoencephalopathy and 36 healthy subjects using enzyme-linked immunosorbent assay (ELISA).
Results:
The activity of respiratory chain complex I was 5.6∓2.4 U/ml in patients with heroin spongiform leucoencephalopathy, significantly higher than that in the normal subjects (4.2∓2.1 U/ml, t=2.634, P<0.05).
Conclusion:
In patients with heroin spongiform leucoencephalopathy, mitochondrial dysfunction results in energy metabolism disorder to cause extensive demyelination of the cerebral white matter. Respiratory chain complex I deficiency of the mitochondria plays a significant role in the pathogenesis of heroin spongiform leucoencephalopathy.
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