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Related Experiment Video

Updated: May 7, 2026

Fabrication Procedures and Birefringence Measurements for Designing Magnetically Responsive Lanthanide Ion Chelating Phospholipid Assemblies
09:38

Fabrication Procedures and Birefringence Measurements for Designing Magnetically Responsive Lanthanide Ion Chelating Phospholipid Assemblies

Published on: January 3, 2018

Modeling, characterizing, and accommodating static birefringence in circular and linear dichroism spectroscopy.

Julie M DiNitto1, John M Kenney

  • 1East Carolina University, Department of Physics, Greenville, NC 27858 USA.

Applied Spectroscopy
|September 27, 2013
PubMed
Summary

Photoelastic modulators (PEMs) in CD and LD spectroscopy have inherent static birefringence. A new model corrects for this, improving measurement accuracy for chiral and linear molecules.

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Last Updated: May 7, 2026

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Published on: June 5, 2019

Area of Science:

  • Spectroscopy
  • Optical Physics
  • Materials Science

Background:

  • Circular dichroism (CD) and linear dichroism (LD) spectroscopy are essential for analyzing molecular structure.
  • Photoelastic modulators (PEMs) are key components in CD/LD spectrometers, inducing birefringence via high-tension (HT) voltage.
  • PEMs possess inherent static birefringence even at zero HT, potentially affecting measurement accuracy.

Purpose of the Study:

  • To characterize the static birefringence in PEMs.
  • To investigate the impact of static birefringence on CD and LD measurements.
  • To develop and validate a novel model accounting for PEM static birefringence.

Main Methods:

  • Characterization of inherent static birefringence in PEMs.
  • Development of a new mathematical model to correct for static birefringence.
  • Experimental validation using CD and LD spectroscopy on camphorsulfonic acid and chrysazin.

Main Results:

  • The inherent static birefringence of PEMs was quantified.
  • The developed model effectively compensates for static birefringence.
  • Validated measurements showed improved accuracy for chiral and linear molecules.

Conclusions:

  • Static birefringence in PEMs is a significant factor affecting CD and LD measurements.
  • The novel model provides accurate compensation for PEM static birefringence.
  • This work enhances the reliability of CD and LD spectroscopy for molecular analysis.