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Updated: May 7, 2026

13:21
In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
Published on: November 27, 2016
Creation of a caspase-3 sensing system using a combination of split-GFP and split-intein
Seiji Sakamoto1, Mika Terauchi, Anna Hugo
1Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan. sakamoto@tagen.tohoku.ac.jp hiko@tagen.tohoku.ac.jp.
Abstract:
A genetically encodable caspase-3 sensing system has been created using self-assembling split-GFP, in which a C-terminal fragment is "covalently" cyclized via a caspase-3 substrate sequence mediated by split-intein. The specific cleavage of the cyclic C-terminal fragment by caspase-3 induces the GFP reassembly and fluorescence recovery.

