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Related Experiment Video

Updated: May 7, 2026

Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation
06:29

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Published on: May 2, 2020

Decrease in hyperosmotic stress-induced corneal epithelial cell apoptosis by L-carnitine.

Neeta Khandekar1, Mark D P Willcox, Sharon Shih

  • 1Brien Holden Vision Institute, Sydney, Australia.

Molecular Vision
|September 27, 2013
PubMed
Summary
This summary is machine-generated.

L-carnitine protects human corneal cells from hyperosmotic stress by regulating cell volume and reducing apoptosis. This study highlights L-carnitine

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Biochemistry

Background:

  • Hyperosmotic stress can damage corneal epithelial cells.
  • Cell volume regulation and apoptosis are critical in maintaining corneal health.

Purpose of the Study:

  • To investigate the osmoprotective effects of L-carnitine on human corneal epithelial cells.
  • To assess L-carnitine's impact on cell volume and apoptosis under hyperosmotic conditions.

Main Methods:

  • Human corneal limbal epithelial (HCLE) cells were subjected to isotonic (300 mOsm) and hyperosmotic (500 mOsm) stress.
  • L-carnitine (10 mM) was added to assess its protective role.
  • Apoptosis markers (caspase activity, TNF-α, annexin V/PI staining) and cell volume changes were analyzed.

Main Results:

  • Hyperosmotic stress significantly increased cell shrinkage, death, TNF-α levels, and caspase activity.
  • L-carnitine partially restored cell volume and significantly reduced TNF-α and caspase-9 activity.
  • L-carnitine mitigated hyperosmotic stress-induced cell damage.

Conclusions:

  • L-carnitine demonstrates osmoprotective properties for corneal epithelial cells.
  • L-carnitine effectively regulates cell volume and ameliorates apoptosis induced by hyperosmotic stress.