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Improved genetically-encoded, FlincG-type fluorescent biosensors for neural cGMP imaging

Yogesh Bhargava1, Kathryn Hampden-Smith, Konstantina Chachlaki

  • 1Wolfson Institute for Biomedical Research, University College London London, UK.

Frontiers in Molecular Neuroscience
|September 27, 2013
PubMed
Summary

Researchers improved the FlincG biosensor for better cyclic guanosine monophosphate (cGMP) imaging in neurons. Optimized FlincG variants (FlincG2 and FlincG3) show enhanced fluorescence and cGMP sensing capabilities for cellular studies.

Keywords:
C-type natriuretic peptidecGMPdorsal root gangliongenetically-encoded biosensorhippocampusneuroblastomanitric oxide

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...