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Related Concept Videos

Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

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Related Experiment Video

Updated: May 7, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

NudC deacetylation regulates mitotic progression.

Carol Chuang1, Jing Pan, David H Hawke

  • 1Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, United States of America.

Plos One
|September 27, 2013
PubMed
Summary
This summary is machine-generated.

Nuclear distribution protein C (NudC) deacetylation, regulated by HDAC3, is crucial for proper cell division and chromosome alignment during mitosis. This deacetylation ensures correct spindle function and prevents mitotic abnormalities.

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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
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Last Updated: May 7, 2026

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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Mitosis relies on protein posttranslational modifications, including acetylation.
  • The role of protein acetylation and deacetylation in regulating mitotic progression is not fully understood.
  • Nuclear distribution protein C (NudC), a key regulator of cell division, is known to be acetylated.

Purpose of the Study:

  • To investigate the role of NudC acetylation and deacetylation in mitotic progression.
  • To identify the specific acetylation site on NudC and its functional significance.
  • To explore the potential involvement of histone deacetylase 3 (HDAC3) in NudC deacetylation during mitosis.

Main Methods:

  • Mass spectrometry to identify NudC acetylation sites.
  • Cellular reconstitution assays using wild-type and mutant NudC.
  • Co-immunoprecipitation and knockdown experiments to study protein interactions.
  • Microscopy to analyze mitotic phenotypes and spindle morphology.

Main Results:

  • NudC acetylation decreases during mitosis, with K39 identified as a key acetylation site.
  • Acetylation-defective NudC (K39R) rescues mitotic defects, while acetylation-mimetic NudC (K39Q) does not, indicating deacetylation is essential.
  • NudC co-localizes and co-immunoprecipitates with HDAC3.
  • HDAC3 knockdown or inhibition increases NudC acetylation.
  • NudC and HDAC3 knockdown result in overlapping mitotic abnormalities, including spindle collapse and chromosome misalignment.

Conclusions:

  • NudC deacetylation is critical for proper mitotic progression and spindle function.
  • HDAC3 is likely involved in the deacetylation of NudC during mitosis.
  • Dysregulation of NudC acetylation/deacetylation contributes to mitotic abnormalities and chromosome segregation errors.