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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

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DNA detection using origami paper analytical devices.

Karen Scida1, Bingling Li1, Andrew D Ellington1

  • 1Department of Chemistry and Biochemistry, Center for Nano- and Molecular Science and Technology, The University of Texas at Austin, 105 E. 24th St., Stop A5300, Austin, TX, 78712-1224, USA.

Analytical Chemistry
|September 28, 2013
PubMed
Summary
This summary is machine-generated.

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We developed a novel origami-based paper analytical device (oPAD) for sensitive DNA detection. This fluorescence-based method achieves low detection limits for single-stranded DNA (ssDNA) and functions in logic gate systems.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Materials Science

Background:

  • Paper analytical devices (PADs) offer low-cost, portable platforms for diagnostics.
  • Origami engineering enables complex 3D fluidic control on paper substrates.
  • Fluorescence-based detection provides high sensitivity for biomolecule quantification.

Purpose of the Study:

  • To demonstrate a hybridization-induced fluorescence detection method for DNA using an origami-based paper analytical device (oPAD).
  • To develop and validate a competitive assay for single-stranded DNA (ssDNA) detection on the oPAD.
  • To evaluate the oPAD's performance in logic gate (OR/AND) systems.

Main Methods:

  • Fabrication of a 3D oPAD using wax printing and controlled heating for hydrophilic channels and hydrophobic barriers.

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  • Development of a competitive fluorescence assay involving ssDNA analyte, a quencher-labeled ssDNA, and a fluorophore-labeled ssDNA probe.
  • Measurement of fluorescence intensity in response to varying ssDNA analyte concentrations.

    • Main Results:

    • Achieved a linear fluorescence response correlated with ssDNA analyte concentration.
    • Extrapolated limit of detection (LOD) below 5 nM for ssDNA.
    • Demonstrated high sensitivity with a relative standard deviation (RSD) as low as 3% for the assay.
    • Successfully implemented the oPAD in OR/AND logic gate detection systems.

    Conclusions:

    • The developed oPAD provides a sensitive and reliable platform for fluorescence-based DNA detection.
    • The 3D oPAD architecture facilitates controlled fluidics for competitive hybridization assays.
    • The system's adaptability to logic gate operations highlights its potential for complex biosensing applications.