Related Experiment Video

Updated: May 7, 2026

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
09:12

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

Coordinated role of voltage-gated sodium channels and the Na+/H+ exchanger in sustaining microglial activation during

Muhammad M Hossain1, Patricia K Sonsalla, Jason R Richardson

  • 1Department of Environmental and Occupational Medicine and Environmental and Occupational Health Sciences Institute, Rutgers Robert Wood Johnson Medical School, Piscataway, NJ, USA.

Toxicology and Applied Pharmacology
|September 28, 2013
PubMed

Keywords:
(Na(+))(i)2,7-Dichlorofluorescin diacetate4′,6-Diamidino-2-phenylindoleDAPIGAPDHH(2)DCFDAH(2)O(2)KRHBKrebs–Ringer–HEPES bufferLPSLipopoysaccharideMEMMicrogliaNADPH oxidaseNHENa(+)/H(+) exchangerNeurodegenerationPBSROSSodium ChannelSodium hydrogen exchangerTNFαTTBSTTXTween 20 Tris buffered salineVGSCglyceraldehyde 3-phosphate dehydrogenasehydrogen peroxideintracellular sodiumlipopolysaccharideminimum essential mediumnicotinamide adenine dinucleotide phosphate-oxidasephosphate-buffered salineqPCRquantitative reverse transcriptase polymerase chain reactionreactive oxygen speciestetrodotoxintumor necrosis factor-αvoltage-gated sodium channels

More Related Videos

Generating and Co-culturing Murine Primary Microglia and Cortical Neurons08:47

Generating and Co-culturing Murine Primary Microglia and Cortical Neurons

Published on: July 26, 2024

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility07:54

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Published on: April 13, 2017

Related Concept Videos

Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
The Role of Ion Channels in Neuronal Computation01:19

The Role of Ion Channels in Neuronal Computation

A postsynaptic neuron usually receives numerous impulses from several other presynaptic neurons. The axon hillock of the postsynaptic neuron integrates all these signals and determines the likelihood of firing an action potential.
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential.
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Ion Channels01:19

Ion Channels

The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow specific...
Nervous Tissue: Glial Cells01:31

Nervous Tissue: Glial Cells

Glia, or neuroglia, are vital support cells that assist neurons in their functions. The term "glia" originates from the Greek word for "glue," reflecting their role in holding the nervous system together. These cells can be categorized into six types: four in the central nervous system (CNS) and two in the peripheral nervous system (PNS).
The CNS glial cell includes the astrocytes, the oligodendrocytes, the microglia, and the ependymal cells.
Astrocytes are star-shaped glial cells that interact...
Inflammation01:38

Inflammation

Overview