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Updated: May 7, 2026

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

Published on: July 23, 2017

Embedding islet in a liquid scaffold increases islet viability and function.

Azadeh Hosseini-Tabatabaei¹, Reza Baradar Jalili, Ryan Hartwell

¹Department of Surgery, University of British Columbia, Vancouver, British Columbia, Canada.

Canadian Journal of Diabetes

|September 28, 2013

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A new crosslinked collagen matrix (CCM) improves islet transplant survival and function. Local indoleamine-2,3-dioxygenase (IDO) delivery via engineered fibroblasts within the CCM enhances islet viability without toxicity, offering a promising approach for type 1 diabetes treatment.

Area of Science:

Biomaterials Science
Regenerative Medicine
Immunology

Background:

Islet transplantation is a key strategy for type 1 diabetes, but faces challenges like donor shortage, poor graft survival, and immunosuppressant toxicity.
Previous fibroblast-populated collagen matrices (CM) improved islet function but suffered from degradation and contraction.
Systemic immunosuppression poses risks; local immunosuppression using indoleamine-2,3-dioxygenase (IDO) is an alternative.

Purpose of the Study:

To develop and evaluate a novel bioengineered crosslinked collagen matrix (CCM) for enhanced islet transplantation.
To assess the viability and function of islets encapsulated within fibroblast-populated CCM (FP-CCM).
To investigate the efficacy and safety of local IDO delivery using IDO-expressing fibroblasts within the FP-CCM to reduce immunosuppression.

Keywords:

amino-indole 2,3– dioxygénase crosslinked collagen matrix greffe d’îlots indoleamine-2,3-dioxygenase islet transplantation matrice collagène réticulée

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Last Updated: May 7, 2026

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

Published on: July 23, 2017

Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating

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Published on: June 23, 2018

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Published on: May 25, 2021

Main Methods:

Development of a crosslinked collagen matrix (CCM) to improve biomimetic properties and stability.
Encapsulation of islets within fibroblast-populated CCM (FP-CCM).
Transduction of fibroblasts with a lentiviral vector for indoleamine-2,3-dioxygenase (IDO) expression and assessment of islet viability in vitro and in vivo.

Main Results:

Islets encapsulated within the fibroblast-populated CCM (FP-CCM) demonstrated significantly improved survival and function.
Local delivery of IDO via lentiviral transduction in fibroblasts integrated into the CCM was non-toxic to the embedded islets.
The engineered CCM provided a stable and supportive microenvironment for transplanted islets.

Conclusions:

The novel fibroblast-populated crosslinked collagen matrix (FP-CCM) with local IDO delivery represents a promising strategy to enhance islet transplant outcomes.
This approach offers a potential solution to overcome key limitations in islet transplantation, including graft survival and the need for systemic immunosuppression.
Further research into this bioengineered construct could pave the way for improved therapies for type 1 diabetes.