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Related Experiment Video

Updated: May 7, 2026

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

Published on: September 5, 2013

Altered sphingoid base profiles predict compromised membrane structure and permeability in atopic dermatitis.

Nicolas Loiseau¹, Yasuko Obata, Sam Moradian

¹Department of Dermatology, School of Medicine, University of California, San Francisco, San Francisco, CA, USA; Veteran Affairs Medical Center, San Francisco, CA, USA; Northern California Institute for Research and Education, San Francisco, CA, USA; Integrative Toxicology and Metabolism, Pôle de Toxicologie Alimentaire, Laboratoire de Pharmacologie et Toxicologie, Institut National de la Recherche Agronomique INRA UR66, Toulouse, France.

Journal of Dermatological Science

|September 28, 2013

Summary

Altered sphingoid base levels in the skin barrier affect membrane structure and permeability. This finding suggests a potential role for sphingoid bases in atopic dermatitis (AD) barrier dysfunction.

Keywords:

2,5-carboxyfluorescein AD Atopic dermatitis Barrier CFC Cer Ceramide DSC FFA LV SC SDS Sa So Sphingosine atopic dermatitis ceramide differential scanning calorimetry free fatty acids sodium dodecyl sulfate sphinganine sphingosine stratum corneum unilamellar vesicles

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Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Published on: September 26, 2019

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Last Updated: May 7, 2026

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

Published on: September 5, 2013

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Published on: September 26, 2019

Area of Science:

Dermatology
Biochemistry
Materials Science

Background:

Ceramide hydrolysis in the stratum corneum (SC) produces sphingoid bases and free fatty acids (FFA).
FFA are crucial for epidermal permeability barrier function.
The role of sphingoid bases in barrier homeostasis and their alterations in atopic dermatitis (AD) are not well understood.

Purpose of the Study:

To investigate sphingoid base alterations in the SC of normal versus AD mouse models.
To determine if altered sphingoid base levels or ratios impact membrane stability and structure.

Main Methods:

Utilized unilamellar vesicles (LV) mimicking SC lipids with varying sphingoid base ratios.
Measured membrane stability via carboxyfluorescein release.
Performed differential scanning calorimetry (DSC) for thermal analysis of LV.

Main Results:

LV with AD-associated sphingoid base levels exhibited altered membrane permeability compared to normal LV.
DSC revealed reduced orthorhombic structures in AD-LV, indicating less tightly packed lamellar arrangements.

Conclusions:

Sphingoid base composition significantly influences SC lamellar membrane architecture.
Abnormal sphingoid base profiles may contribute to the skin barrier defects observed in AD.