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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Highly efficient bienzyme functionalized nanocomposite-based microfluidics biosensor platform for biomedical

Md Azahar Ali¹, Saurabh Srivastava, Pratima R Solanki

¹1] Department of Science and Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, CSIR-National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India [2] Indian Institute of Technology Hyderabad, Ordnance Factory Estate, Yeddumailaram, Hyderabad, Andhra Pradesh 502205, India.

Scientific Reports

|September 28, 2013

Summary

This summary is machine-generated.

Researchers developed a novel microfluidics nanobiochip using nickel oxide nanoparticles and carbon nanotubes for rapid, sensitive biomolecule detection in small samples.

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Area of Science:

Biomedical Engineering
Nanotechnology
Biosensors

Background:

Microfluidic devices offer advantages for biological assays.
Nanomaterials enhance biosensor performance.

Purpose of the Study:

To fabricate and characterize a novel microfluidics nanobiochip.
To evaluate its performance for biomolecule detection.

Main Methods:

Fabrication of a nanocomposite using nickel oxide nanoparticles (nNiO) and multiwalled carbon nanotubes (MWCNTs).
Integration of the nanocomposite with polydimethylsiloxane (PDMS) microchannels via photolithography.
Functionalization with a bienzyme (cholesterol oxidase and cholesterol esterase).
Characterization using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM).

Main Results:

Successful fabrication of the microfluidics nanobiochip.
XPS confirmed amide bond formation between the nNiO-MWCNT composite and the bienzyme (9.3% carboxyl group utilization).
The nanobiochip demonstrated high sensitivity (2.2 mA/mM/cm²), good reproducibility, and selectivity.

Conclusions:

The developed microfluidics nanobiochip is a promising platform for rapid biomolecule detection.
It offers a low-cost solution for analyzing minute biological samples.
The integration of nNiO-MWCNT nanocomposite enhances biosensor capabilities.