High-throughput oenomics: shotgun polyphenomics of wines

Area of Science:

• Analytical Chemistry
• Food Science
• Metabolomics

Background:

• Red wine composition is complex, containing numerous organic compounds like polyphenols.
• Understanding wine quality and authenticity is crucial for consumers and producers.
• Existing analytical methods for wine polyphenols can be time-consuming.

Purpose of the Study:

• To develop a fast and comprehensive method for red wine polyphenol characterization.
• To establish the first shotgun polyphenomics approach for wine analysis.
• To compare the new method's efficiency against existing techniques.

Main Methods:

• Development of an original, fast shotgun polyphenomics method for red wine.
• Utilized ultra-high performance liquid chromatography-electrospray ionization-quadrupole-time-of-flight-mass spectrometry (UHPLC-ESI-Q-TOF-MS).
• Employed direct injection-electrospray ionization-mass spectrometry (DI-ESI-MS) for comparison.

Main Results:

• The new method identified 103 compounds in a rapid 2-minute analysis.
• Significantly more information was obtained compared to previous fast methods like DI-ESI-MS.
• Preliminary analysis using a selected ion ratio revealed distinct spectral differences among Pinot Noir, Merlot, and Syrah wines.

Conclusions:

• The developed shotgun polyphenomics method offers a highly efficient approach for red wine analysis.
• This technique provides deeper insights into wine quality and authenticity.
• The method shows potential for differentiating wine varietals based on their polyphenol profiles.