Functional characteristics of connective tissue growth factor on human tenon's capsule fibroblast

To explore the characteristic of connective tissue growth factor (CTGF) on the phenotype transition, extracellular matrix (ECM) synthesis and proliferation of human Tenon's capsule fibroblasts (HTFs).

HTFs were obtained from patients during cataract surgery and induced by CTGF (1 to 100 µg/L). Western blot and immunofluorescence were performed to observe the expression of alpha smooth muscle actin (α-SM-actin) protein. The levels of mRNAs were quantified by real-time PCR. Col I and FN expression at both protein and RNA levels were tested after induction by CTGF and transforming growth factor β (TGF-β), respectively. Statistical significance was assumed if p < 0.05.

CTGF upregulated the expression of α-SM-actin in cultured HTFs. Its maximum effect at protein level attained under the optimal concentration of 50 μg/L at the peak time of 48 hours, though still weaker than the effect of TGF-β1 (10 μg/L, p < 0.05). The expression of Col I and FN at both protein and mRNA levels was elevated by the induction of CTGF (50 μg/L) (p < 0.01) and TGF-β1 (10 μg/L) (p < 0.05), while CTGF (50 μg/L) showed a greater effect than the latter (p < 0.05). CTGF (1 to100 μg/L) increased the proliferation of HTFs significantly (p < 0.05).

CTGF induced the phenotype transition of HTFs individually and significantly promoted their proliferation. Moreover, it promoted ECM synthesis, thus demonstrating its role as a crucial factor in fibrosis. Thus, CTGF could potentially be a safer and more efficient target than TGF-β at suppressing scar formation after filtering surgery.