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Related Experiment Video

Updated: May 7, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

G1 compartmentalization and cell fate coordination.

Stephen Dalton¹

¹Department of Biochemistry and Molecular Biology, Paul D. Coverdell Center for Biomedical and Health Sciences, The University of Georgia, 500 DW Brooks Drive, Athens, GA 30602, USA.

|October 1, 2013

Summary

Cell cycle progression influences stem cell fate. Cyclin D in late G1 restricts TGF-β signaling, switching pluripotent stem cells from endoderm to neuroectoderm differentiation.

Area of Science:

Stem cell biology
Cell cycle regulation
Developmental biology

Background:

Pluripotent stem cells can differentiate into various cell types.
Cell signaling pathways, like TGF-β, regulate differentiation.
The cell cycle's role in lineage specification is not fully understood.

Purpose of the Study:

To investigate how the cell cycle influences the differentiation potential of pluripotent stem cells.
To determine the role of cyclin D in TGF-β-dependent differentiation.
To understand the coordination between cell cycle, signaling, and lineage choice.

Main Methods:

Utilized pluripotent stem cells.
Manipulated cell cycle phases (G1).
Assessed TGF-β signaling pathway activity (Smad2/3).

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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

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Published on: December 5, 2017

Analyzed lineage specification (endoderm vs. neuroectoderm).

Main Results:

Early G1 phase is permissive for TGF-β-induced endoderm differentiation.
Cyclin D in late G1 restricts Smad2/3 activity.
This restriction causes a switch in cell potential from endoderm to neuroectoderm.

Conclusions:

Cell cycle phase, specifically cyclin D activity in late G1, is a critical determinant of stem cell lineage potential.
Coordination between cell cycle progression and signaling pathways like TGF-β governs differentiation outcomes.
Findings provide a mechanistic link between cell cycle control and developmental decisions in stem cells.