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Presence of immunoreactive endothelin in human milk
H C Lam1, K Takahashi, M A Ghatei
1Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
FEBS Letters
|February 12, 1990
Abstract:
Endothelin-like immunoreactivity was detected in human milk at a concentration of 6.8 +/- 1.6 pmol/l (mean +/- SEM; n = 16) using a highly sensitive radioimmunoassay. Gel filtration and fast protein liquid chromatography (FPLC) verified the identity of the endothelin. FPLC revealed 4 peaks, one eluting just after the void volume, and the other three in the positions of endothelin-1, -2, and -3, respectively.