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Updated: May 7, 2026
06:26
Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
Published on: June 19, 2017
Progress in microRNA delivery
Yu Zhang1, Zaijie Wang, Richard A Gemeinhart
1Department of Biopharmaceutical Sciences, University of Illinois, Chicago, IL 60612-7231, USA.
Summary
MicroRNAs (miRNAs) regulate gene expression and disease. Nonviral delivery systems are crucial for overcoming challenges in miRNA-based therapies, paving the way for clinical applications.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- MicroRNAs (miRNAs) are non-coding RNAs regulating gene expression post-transcriptionally.
- miRNAs bind to messenger RNAs (mRNAs), affecting gene expression networks.
- Dysregulation of miRNAs is implicated in various diseases, highlighting therapeutic potential.
Purpose of the Study:
- To review advances in nonviral miRNA delivery systems.
- To discuss strategies for overcoming delivery challenges in miRNA therapeutics.
- To provide a perspective on the future of miRNA-based therapies.
Main Methods:
- Review of recent scientific literature on miRNA mechanisms and delivery.
- Analysis of viral and nonviral delivery vector strategies.
- Discussion of limitations and potential solutions for in vivo miRNA delivery.
Main Results:
- Nonviral delivery systems offer promising alternatives to viral vectors for miRNA therapeutics.
- Key challenges include in vivo stability, biodistribution, and off-target effects.
- Advances in nonviral systems aim to improve efficiency and reduce toxicity.
Conclusions:
- Nonviral miRNA delivery is essential for advancing miRNA-based therapies.
- Overcoming delivery hurdles is critical for clinical translation.
- Future research should focus on optimizing nonviral systems for safety and efficacy.
Keywords:
1,2-Di-O-octadecenyl-3-trimethylammonium propane2′-MOE2′-Me2′-methoxyethyl2′-methylAMOAPPBBBCNSCSCDDABDGCR8DNADOTMADiGeorge syndrome critical region geneFANAGC4HCVHuman diseaseIRLACLNALPHMicroRNANLENSCLCNonviral deliveryODNPEIPNAPURISCRNARNA induced silencing complex: scFv, single-chain variable fragmentSmall RNA deliveryTEPA-PCLTPGSUTRVEGFamyloid precursor proteinanti-miRNA oligonucleotidesbase pairsblood–brain barrierbpcancer stem cellcentral nervous systemd-alpha-tocopheryl polyethylene glycol 1000 succinatedeoxyribonucleic aciddicetyl phosphate-tetraethylenepentamine-based polycation liposomesdimethyldioctadecylammonium bromidefluorine derivatives nucleic acidhepatitis C virusiNOPionizing radiationliposome-hyaluronic acidlocked nucleic acidlung adenocarcinomamiRNAmiRNA therapeuticsmicroRNAnanotransporter interfering nanoparticle-7neutral lipid emulsionnon-small cell lung cancerntnucleotidesoligodeoxynucleotidespeptide nucleic acidsphage identified internalizing scFvs that target tumor sphere cellspolyethyleneiminepolyurethaneribonucleic acidsiRNAsmall interfering RNAuntranslated regionsvascular endothelial growth factor
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