Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A universal 6iL/E4 culture system for deriving and maintaining embryonic stem cells across mammalian species.

Cell research·2026
Same author

Cognitive function depends upon <i>Satb2</i> gene dosage in cortical projection neurons.

bioRxiv : the preprint server for biology·2026
Same author

Generation of Chimera-Competent Avian iPSCs Using Defined Transcription Factors.

Cells·2026
Same author

Expansion and CAR engineering of granulocyte-monocyte progenitors for cellular immunotherapy.

Cell·2026
Same author

A nucleus accumbens-projecting prefrontal cortex circuit underlies chronic social stress-induced depression-like behaviors.

Translational psychiatry·2026
Same author

Carboxymethyl chitosan/sodium alginate-based dynamic organic nanocomposite hydrogel for local tumoral targeted therapy.

Carbohydrate polymers·2026

Related Experiment Video

Updated: May 7, 2026

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Published on: July 11, 2019

Induced neural stem cells generated from rat fibroblasts.

Guangjun Xi1, Pingfang Hu, Cunye Qu

  • 1Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Cell and Neurobiology, University of Southern California, Los Angeles, CA 90033, USA; Department of Neurology, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi 214023, China.

Genomics, Proteomics & Bioinformatics
|October 1, 2013
PubMed
Summary

Researchers developed a method to maintain pure neural stem (NS) cells and generate induced NS (iNS) cells from fibroblasts. This breakthrough offers new possibilities for regenerative medicine and stem cell research.

Keywords:
FibroblastsInduced neural stem cellsInduced pluripotent stem cellsRatStem cell self-renewal

More Related Videos

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
08:26

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

Published on: May 14, 2015

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
13:58

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Published on: July 29, 2015

Related Experiment Videos

Last Updated: May 7, 2026

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Published on: July 11, 2019

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
08:26

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

Published on: May 14, 2015

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
13:58

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Published on: July 29, 2015

Area of Science:

  • Stem Cell Biology
  • Neuroscience
  • Regenerative Medicine

Background:

  • Maintaining pure tissue-specific stem cells, like neural stem (NS) cells, is challenging.
  • Existing methods for embryonic stem (ES) cell culture are not directly applicable to all stem cell types.

Purpose of the Study:

  • To establish methods for the maintenance of pure neural stem (NS) cells.
  • To generate induced neural stem (iNS) cells from somatic cells.

Main Methods:

  • Utilized a cocktail of cytokines and small molecules for cell culture.
  • Maintained primitive NS cells derived from mouse ES cells and rat embryos.
  • Generated iNS cells from rat fibroblasts via forced expression of Oct4, Sox2, and c-Myc transcription factors.

Main Results:

  • Successfully maintained primitive neural stem (NS) cells.
  • Demonstrated the generation of induced neural stem (iNS) cells from rat fibroblasts.
  • Confirmed the long-term maintenance potential of these iNS cells.

Conclusions:

  • A novel method for generating and maintaining induced neural stem (iNS) cells has been developed.
  • This advancement has significant potential implications for regenerative medicine and disease modeling.
  • The findings pave the way for broader applications of induced pluripotent stem cell technology.