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Renoprotective effect of ramulus mori polysaccharides on renal injury in STZ-diabetic mice
Chao Guo1, Tao Liang, Qiaoling He
1Guigang City People's Hospital, The Eighth Affiliated Hospital of Guangxi Medical University, Guigang, Guangxi 537100, PR China.
Abstract:
Recently, increasing evidences have suggested that inflammatory stress is markedly occurred in the impaired tissue. Thus, the streptozotocin (STZ)-induced diabetic mice were used to investigate the potential renoprotective effect of ramulus mori polysaccharides (RMP) and to discuss the underlying mechanism. The results from the present study showed that RMP significantly lowered the blood glucose and serum levels of glycosylated protein, cholesterol, urea nitrogen (Urea-N), creatinine (Cr) and 24-hour urine protein, while the albumin content was elevated. Meanwhile, the proinflammatory cytokines such as interleukin-6 (IL-6), interferon-gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) in renal tissue of STZ-lesioned mice were reduced by RMP treatment. Similarly, pathological examination indicated that STZ-induced renal injury was effectively mitigated. In addition, the protein levels of interleukin-1 (IL-1) and interleukin-1 receptor (IL-1R) in renal tissue were notably decreased. Moreover, the endogenous expressions of phosphorylated-IκB (p-IκB) and nuclear factor-kappa B (NF-κB) were down-regulated, respectively. Together, these findings revealed that RMP treatment effectively attenuated STZ-induced cytotoxicity in renal tissue, in which RMP-exerted renoprotection was associated with intrarenally debilitating inflammation reaction through blocking the IL-1/NF-κB pathway, thereby maintaining the renal homeostasis.
Insights
Ramulus mori polysaccharides (RMP) protect against kidney damage in diabetic mice by reducing inflammation. RMP treatment lowers blood glucose and inflammatory markers, preserving renal homeostasis.
Area of Science:
- Nephrology
- Immunology
- Pharmacology
Background:
- Inflammatory stress is implicated in tissue damage, particularly in diabetic nephropathy.
- Diabetic nephropathy is a significant complication of diabetes mellitus, leading to kidney impairment.
Purpose of the Study:
- To investigate the renoprotective effects of Ramulus Mori Polysaccharides (RMP) in streptozotocin (STZ)-induced diabetic mice.
- To elucidate the underlying mechanisms of RMP's renoprotective action, focusing on inflammatory pathways.
Main Methods:
- Induction of diabetes in mice using streptozotocin (STZ).
- Administration of RMP to STZ-induced diabetic mice.
- Assessment of renal function markers (blood glucose, serum creatinine, urine protein, albumin).
- Measurement of inflammatory cytokines (IL-6, IFN-γ, TNF-α, IL-1, IL-1R) and signaling pathway proteins (p-IκB, NF-κB) in renal tissue.
Main Results:
- RMP significantly reduced blood glucose, glycosylated protein, cholesterol, urea nitrogen, and creatinine levels.
- RMP treatment decreased pro-inflammatory cytokines (IL-6, IFN-γ, TNF-α, IL-1, IL-1R) and inhibited the IL-1/NF-κB pathway in renal tissue.
- Pathological examination confirmed mitigation of STZ-induced renal injury, with RMP improving albumin levels and reducing urine protein.
- RMP down-regulated the expression of phosphorylated-IκB (p-IκB) and nuclear factor-kappa B (NF-κB).
Conclusions:
- RMP exhibits significant renoprotective effects in STZ-induced diabetic mice.
- RMP attenuates renal cytotoxicity by suppressing intrarenal inflammation via the IL-1/NF-κB pathway.
- RMP treatment helps maintain renal homeostasis in the context of diabetic kidney injury.
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