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A simplified and cost-effective method combining real-time PCR and pyrosequencing for detection of aac(6')-Ib-cr gene

Thomas Guillard1, Nicolas Fontaine, Anne Limelette

  • 1CHU Reims, Hôpital Robert Debré, Laboratoire de Bactériologie-Virologie-Hygiène, F-51092 Reims, France; UFR Médecine, EA 4687 ERA, SFR CAP-Santé, Université de Reims Champagne-Ardenne, F-51092 Reims, France.

Journal of Microbiological Methods
|October 1, 2013
PubMed

Keywords:
Plasmid-mediated quinolone resistancePyrosequencingReal-time PCRSYBR Greenaac(6′)-Ib-cr

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...