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06:59
A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior
Published on: May 21, 2020
Targeted mutagenesis tools for modelling psychiatric disorders
1Max Planck Institute of Psychiatry, Molecular Neurogenetics, Kraepelinstrasse 2-10, 80804, Munich, Germany, deussing@mpipsykl.mpg.de.
Cell and Tissue Research
|October 1, 2013
Summary
Gene targeting in mice has revolutionized neurobiology and psychiatric disorder research. Advanced tools like conditional mutagenesis and humanized mice promise more accurate disease modeling and broader genome engineering applications.
Area of Science:
- Genetics
- Neuroscience
- Genomics
Background:
- Gene targeting principles emerged in the 1980s, enabling precise mouse genome engineering.
- Genetic mouse models are crucial for understanding neurobiology and neuropsychiatric disorders.
- Technological advancements, including site-specific recombinases, allow for conditional mutagenesis with high resolution.
Purpose of the Study:
- To review the evolution and impact of genetic mouse models in neurobiological and psychiatric research.
- To highlight advancements in gene targeting technologies for improved disease modeling.
- To discuss future challenges and opportunities in psychiatric disorder modeling using genetic tools.
Main Methods:
- Review of historical developments in gene targeting and mouse genetics.
- Discussion of technological innovations like conditional mutagenesis and site-specific nucleases.
- Analysis of large-scale genome annotation projects and resource development.
Main Results:
- Genetic mouse models have significantly advanced the understanding of neurobiological concepts and psychiatric disorders.
- Conditional mutagenesis provides high spatial and temporal control over gene function.
- Large-scale genomic efforts have accelerated the development and dissemination of mouse genetic tools.
Conclusions:
- Genetic mouse models are indispensable for psychiatric research, with ongoing improvements enhancing disease mechanism comprehension.
- Future challenges include developing more appropriate models for complex psychiatric disorders.
- Emerging technologies like humanized mice and site-specific nucleases offer improved modeling capabilities and broader genome engineering potential across organisms.
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