Multiplexed bioactive paper based on GO@SiO2@CeO2 nanosheets for a low-cost diagnostics platform

Area of Science:

• Materials Science
• Nanotechnology
• Biochemistry

Background:

• Peroxidases are crucial enzymes in biochemical assays.
• Developing stable and efficient peroxidase mimetics is essential for diagnostic applications.
• Graphene oxide and cerium oxide have shown promise in catalytic applications.

Purpose of the Study:

• To synthesize and characterize Graphene oxide@SiO2@CeO2 hybrid nanosheets (GSCs).
• To evaluate the intrinsic peroxidase-like activity of GSCs.
• To develop a reagentless bioactive paper for simultaneous detection of multiple analytes.

Main Methods:

• Wet-chemical synthesis of Graphene oxide@SiO2@CeO2 hybrid nanosheets.
• Characterization using Transmission Electron Microscopy (TEM), Fourier-Transform Infrared Spectroscopy (FTIR), and X-ray Photoelectron Spectroscopy (XPS).
• Colorimetric assays for evaluating peroxidase activity and detecting biomarkers.

Main Results:

• Successful synthesis of Graphene oxide@SiO2@CeO2 hybrid nanosheets.
• GSCs exhibited significant intrinsic peroxidase activity, attributed to the synergistic effect of graphene oxide and CeO2.
• A reagentless bioactive paper integrated with GSCs demonstrated simultaneous detection of glucose, lactate, uric acid, and cholesterol.

Conclusions:

• Graphene oxide@SiO2@CeO2 hybrid nanosheets are effective peroxidase mimetics.
• The developed bioactive paper offers a novel platform for multiplexed biomarker detection.
• GSCs show potential as a cost-effective and stable alternative to traditional peroxidases in clinical settings.