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High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
Published on: August 11, 2014
Gene expression analysis of Salmonella enterica SPI in macrophages indicates differences between serovars that induce
Ariel Imre1, Agnes Bukovinszki, Margaret A Lovell
1School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom; CEVA-Phylaxia Biotechnology Europe, Szállás utca 5, 1107 Budapest, Hungary.
Abstract:
Global gene expression of the invasive Salmonella serovars S. Enteritidis and S. Typhimurium, and the less-invasive S. Infantis and S. Hadar was studied during infection of a chicken macrophage cell line. Major functional gene groups responsible for intracellular physiological changes were regulated similarly in all four serovars. However, SPI1 and SPI4 genes of S. Enteritidis and S. Typhimurium were strongly repressed in the macrophages whereas S. Infantis, S. Hadar and other similar serovars maintained up-regulation of these gene sets. This phenomenon may explain some of the biological differences between invasive and non-invasive Salmonella serovars.
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