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Related Concept Videos

Lipid-derived Compounds in the Human Body01:31

Lipid-derived Compounds in the Human Body

Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various  biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin, delayed...
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Surface Membrane Barriers

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Lipids as Anchors

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Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

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Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
10:51

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Published on: July 14, 2017

Acyl-CoA binding protein and epidermal barrier function.

Maria Bloksgaard1, Ditte Neess1, Nils J Færgeman1

  • 1Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark.

Biochimica Et Biophysica Acta
|October 2, 2013
PubMed
Summary
This summary is machine-generated.

Acyl-CoA binding protein (ACBP) is crucial for skin barrier function. Its absence in mice results in compromised epidermal barrier, increased water loss, and altered skin lipids.

Keywords:
Acyl-CoA binding proteinCeramideEpidermal barrierMonoalkyl–diacyl-glycerolStratum corneumVery long chain fatty acids

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Area of Science:

  • Biochemistry
  • Dermatology
  • Molecular Biology

Background:

  • Acyl-CoA binding protein (ACBP) is a conserved intracellular protein binding acyl-CoA esters.
  • Its precise function is unknown, but it's implicated in lipid transport.
  • ACBP is highly expressed in the epidermis, particularly in lipid-synthesizing suprabasal layers.

Purpose of the Study:

  • To review current knowledge on ACBP.
  • To focus on ACBP's role in epidermal barrier formation and maintenance.
  • To investigate the consequences of ACBP gene disruption on skin phenotype and barrier function.

Main Methods:

  • Gene targeting to create ACBP knockout mice (ACBP(-/-)).
  • Phenotypic analysis of skin and fur.
  • Measurement of transepidermal water loss (TEWL).
  • Lipidomic analysis of the stratum corneum.

Main Results:

  • ACBP gene disruption caused significant skin and fur abnormalities (alopecia, scaling, greasy fur).
  • ACBP(-/-) mice exhibited a ~50% increase in TEWL, indicating compromised epidermal barrier.
  • Stratum corneum lipid analysis revealed significantly reduced levels of non-esterified very long chain fatty acids in ACBP(-/-) mice.

Conclusions:

  • ACBP plays a critical role in maintaining epidermal barrier integrity.
  • Reduced very long chain fatty acids due to ACBP absence likely underlies the compromised barrier function.
  • ACBP is essential for proper lipid metabolism and skin homeostasis.