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Updated: May 7, 2026

Calibration Procedures for Orthogonal Superposition Rheology
Published on: November 18, 2020
Measurement of plasma viscosity by free oscillation rheometry: imprecision, sample stability and establishment of a
Lise Pedersen1, Erling B Nielsen2, Majbrit K Christensen2
1Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Odense, Denmark lise-pedersen@rsyd.dk.
Background:
Plasma viscosity (PV) is used in the investigation of hyperviscosity syndrome. We have evaluated the performance of a new bench-top free oscillation rheometer, ReoRox from Medirox, and established a new reference range.
Methods:
Performance of the free oscillation rheometry (FOR) method was evaluated examining within-run and between-run imprecision, duration of measurement and operator simplicity. Furthermore, influence of storage on the PV measurements was assessed and a reference range based on plasma from 585 healthy men and women was established.
Results:
Imprecision of the free oscillation rheometer was comparable to other viscometers and performance of the instrument was satisfying. The non-parametrical reference range established was 1.22-1.44 mPa s at 37℃ (2.5th and 97.5th percentiles).
Conclusions:
We have established a FOR PV reference range to support clinical decision-making. Furthermore, we found high pre-analytical stability of the samples and an analytical imprecision comparable to other PV methods.
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