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Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
Spermatogenesis01:22

Spermatogenesis

Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
Sperm Transport01:15

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Gene Regulation During Sporulation01:17

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Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
TGF - β Signaling Pathway01:16

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Evaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization
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Taf7l cooperates with Trf2 to regulate spermiogenesis.

Haiying Zhou1, Ivan Grubisic, Ke Zheng

  • 1Howard Hughes Medical Institute and Department of Molecular and Cell Biology, Li Ka Shing Center for Biomedical and Health Sciences, California Institute for Regenerative Medicine Center of Excellence, University of California, Berkeley, CA 94720.

Proceedings of the National Academy of Sciences of the United States of America
|October 2, 2013
PubMed
Summary
This summary is machine-generated.

TATA-binding protein (TBP)-associated factor 7l (Taf7l) is crucial for male fertility. Its absence causes sterility by impairing gene expression during sperm development, highlighting a new regulatory mechanism.

Keywords:
RNA-seqcontraceptive medicinegene regulationreproduction

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Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Genetics

Background:

  • TATA-binding protein (TBP)-associated factor 7l (Taf7l) and TBP-related factor 2 (Trf2) are essential for gene transcription.
  • Taf7l knockout mice exhibit impaired sperm structure, motility, and fertility.

Purpose of the Study:

  • To investigate the role of Taf7l in male fertility and spermiogenesis.
  • To elucidate the molecular mechanism by which Taf7l regulates gene expression in the testis.

Main Methods:

  • Genome-wide mRNA-sequencing and ChIP-sequencing analyses.
  • Histological examination of testes.
  • Biochemical association studies.

Main Results:

  • Taf7l knockout leads to male sterility and postmeiotic arrest during spermiogenesis.
  • Taf7l ablation affects expression of postmeiotic and metabolic genes.
  • Taf7l and Trf2 coregulate postmeiotic genes, with Taf7l binding to their promoters.
  • Taf7l physically interacts with Trf2 in the testis.

Conclusions:

  • Taf7l is essential for mammalian spermiogenesis and male fertility.
  • Taf7l and Trf2 cooperate to regulate testis-specific gene transcription.
  • This interaction represents a novel mechanism for cell-type-specific transcriptional control.