Related Experiment Video
Updated: May 7, 2026
A Seed Coat Bedding Assay to Genetically Explore In Vitro How the Endosperm Controls Seed Germination in Arabidopsis thaliana
Published on: November 9, 2013
Induction of somatic embryogenesis in gum arabic tree [Acacia senegal (L.) Willd]
Jitendra Singh Rathore1, Manoj K Rai, N S Shekhawat
1Biotechnology Centre, Department of Botany, Jai Narain Vyas University, Jodhpur, 342033 Rajasthan India ; Department of Botany and Biotechnology, L.M. College of Science and Technology, Jodhpur, 342001 Rajasthan India.
Abstract:
Factors affecting somatic embryogenesis from immature cotyledon of gum arabic tree [Acacia senegal (L.) Willd.] were investigated. Induction of somatic embryogenesis was influenced by plant growth regulator concentrations and addition of amino acids in medium. Best induction of somatic embryogenesis was obtained on MS medium supplemented with 0.45 μM 2, 4-D, 2.32 μM Kin and 15 mM L-glutamine. L-glutamine plays a significant role in the maturation of somatic embryos and most of embryos attained maturity only on L-glutamine (15 mM) containing medium. Maximum percent (75.0 ± 2.5) germination of somatic embryos was recorded on medium containing 0.22 μM BAP.
More Related Videos
07:32Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
Published on: June 9, 2015
04:32An Induction System for Clustered Stomata by Sugar Solution Immersion Treatment in Arabidopsis thaliana Seedlings
Published on: February 15, 2019