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The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds
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Published on: February 15, 2011

Laminin Functionalized Biomimetic Nanofibers For Nerve Tissue Engineering.

Radoslaw Junka1, Chandra M Valmikinathan, Dilhan M Kalyon

  • 1Department of Chemistry, Chemical Biology and Biomedical Engineering Stevens Institute of Technology, Hoboken, NJ, 07030.

Journal of Biomaterials and Tissue Engineering
|October 2, 2013
PubMed
Summary

This study developed new biomimetic nanofibrous scaffolds for peripheral nerve repair. Functionalizing poly(caprolactone)-chitosan scaffolds with laminin significantly enhanced Schwann cell attachment and proliferation for nerve regeneration.

Keywords:
ChitosanElectrospun NanofibersLamininNerve Tissue Engineering

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Peripheral nerve injuries with large gaps pose significant regeneration challenges.
  • Current treatments lack suitable grafts and efficient cell delivery methods.
  • Biomimetic scaffolds functionalized with extracellular matrix proteins offer potential therapeutic solutions.

Purpose of the Study:

  • To fabricate and characterize novel poly(caprolactone)-chitosan nanofibrous scaffolds.
  • To functionalize scaffold surfaces with laminin using carbodiimide crosslinking.
  • To evaluate scaffold properties for peripheral nerve tissue engineering.

Main Methods:

  • Fabrication of poly(caprolactone) (PCL) and chitosan blend nanofibrous scaffolds via electrospinning.
  • Surface functionalization of scaffolds with laminin using carbodiimide crosslinking.
  • Assessment of scaffold wettability, mechanical properties, Schwann cell attachment, and proliferation.

Main Results:

  • PCL-chitosan scaffolds exhibited improved wettability for cell attachment compared to neat PCL.
  • Carbodiimide crosslinking enabled higher concentrations of surface-bound laminin.
  • Significantly enhanced Schwann cell proliferation was observed on scaffolds with crosslinked laminin.

Conclusions:

  • PCL-chitosan nanofibrous scaffolds functionalized with crosslinked laminin show promise for nerve tissue engineering.
  • These scaffolds offer improved surface properties and enhanced cell response for peripheral nerve repair.
  • The developed scaffolds represent a versatile substrate for in vivo cell delivery applications.