Green synthesis of silver nanoparticles using Pinus eldarica bark extract

Recently, development of reliable experimental protocols for synthesis of metal nanoparticles with desired morphologies and sizes has become a major focus of researchers. Green synthesis of metal nanoparticles using organisms has emerged as a nontoxic and ecofriendly method for synthesis of metal nanoparticles. The objectives of this study were production of silver nanoparticles using Pinus eldarica bark extract and optimization of the biosynthesis process. The effects of quantity of extract, substrate concentration, temperature, and pH on the formation of silver nanoparticles are studied. TEM images showed that biosynthesized silver nanoparticles (approximately in the range of 10-40 nm) were predominantly spherical in shape. The preparation of nano-structured silver particles using P. eldarica bark extract provides an environmentally friendly option, as compared to currently available chemical and/or physical methods.