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Updated: May 7, 2026

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
Published on: July 3, 2013
GPR48 promotes multiple cancer cell proliferation via activation of Wnt signaling
Yong-Bin Zhu1, Lin Xu, Ming Chen
1Department of Otolaryngology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Abstract:
The key signaling networks regulating cancer cell proliferation remain to be defined. The leucine-rich repeat containing G-protein coupled receptor 48 (GPR48) plays an important role in multiple organ development. In the present study, we investigated whether GPR48 functions in cancer cells using MCF-7, HepG2, NCI-N87 and PC-3 cells. We found that GPR48 overexpression promotes while its knockdown using small interfering RNA oligos inhibits cell proliferation. In addition, Wnt/β-catenin signaling was activated in cells overexpressing GPR48. Therefore, our results indicated that GPR48 activates Wnt/β-catenin signaling to regulate cancer cell proliferation.
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