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Updated: May 7, 2026

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
Published on: August 2, 2018
TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells
C Marconi1, S Peppicelli, F Bianchini
1Departament of Experimental and Clinical Biomedical Sciences, Section of Experimental Pathology and Oncology, University of Florence, Istituto Toscano Tumori, Florence, Italy.
Aim:
Oxygen deprivation leading to hypoxia represents a common feature of advanced solid tumors, able to control several aspects of tumor progression. Indeed, ability to respond to changes in oxygen partial pressure represents a hallmark of malignant cells. Aim of this study is to disclose new pathway of hypoxia-induced tumor cell invasion.
Materials And Methods:
Hs294T human melanoma cells were grown in a gas mixture containing 0.3% O2 and used to evaluate invasion on Matrigel-coated polycarbonate filters mounted in Boyden's chambers, MMP release and expression of inflammatory receptors and their ligands.
Results:
We demonstrate that hypoxia promotes the expression of TNFα receptor 1 (TNFαR1) able to drive a higher ability to penetrate Matrigel-coated filters of Hs294T human melanoma cells, an effect does not mediated by hypoxia-inducible factor (HIF)-1α.
Conclusion:
Expression of inflammatory cytokine receptors in hypoxic human melanoma cells might provide a new target for improving strategies against local and distant tumor cell diffusion.
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