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Related Experiment Videos

Taurocholate transport by basolateral plasma membrane vesicles isolated from developing rat liver.

F J Suchy, S M Courchene, B L Blitzer

    The American Journal of Physiology
    |June 1, 1985
    PubMed
    Summary

    Taurocholate transport is significantly reduced in young rats compared to adults. This decreased sodium-coupled uptake in suckling rat liver vesicles is due to a lower maximum transport rate (Vmax).

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    Area of Science:

    • Hepatology
    • Membrane Transport
    • Neonatal Physiology

    Background:

    • Bile acid transport is crucial for liver function.
    • Understanding developmental changes in transport mechanisms is important for neonatal health.

    Purpose of the Study:

    • To characterize taurocholate transport in suckling rat liver.
    • To compare the kinetics of taurocholate uptake in suckling versus adult rats.

    Main Methods:

    • Preparation of basolateral plasma membrane vesicles from suckling and adult rat livers using Percoll gradients.
    • Assay of taurocholate uptake under sodium (Na+) and potassium (K+) gradients.
    • Kinetic analysis of taurocholate transport.

    Main Results:

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  • Basolateral membrane vesicles from suckling rats showed similar protein yield and marker enzyme enrichment to adult vesicles.
  • Sodium-dependent taurocholate uptake exhibited an 'overshoot' phenomenon in both age groups, but with a markedly decreased initial rate and peak accumulation in suckling rats.
  • Kinetic studies revealed a significantly lower Vmax for taurocholate uptake in suckling rat vesicles (less than half the adult rate).

    • Conclusions:

    • Taurocholate transport is less efficient in suckling rat liver compared to adults.
    • The reduced transport capacity in neonates is primarily attributed to a lower maximal transport rate (Vmax) rather than altered sodium gradient dissipation.