Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 7, 2026

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
07:07

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Published on: January 12, 2017

In vitro reparative dentin: a biochemical and morphological study.

G Teti1, V Salvatore, A Ruggeri

  • 1University of Bologna. mirella.falconi@unibo.it.

European Journal of Histochemistry : EJH
|October 3, 2013
PubMed
Summary

Researchers successfully simulated reparative dentinogenesis using human dental pulp cells. This study provides insights into odontoblast differentiation and dentin matrix mineralization for future investigations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early multimodal behavioral cues in autism: a micro-analytical exploration of actions, gestures and speech during naturalistic parent-child interactions.

International journal of clinical and health psychology : IJCHP·2026
Same author

Indications for haematopoietic cell transplantation and CAR-T for haematological diseases, solid tumours and immune disorders: 2025 EBMT practice recommendations.

Bone marrow transplantation·2025
Same author

CC486 as a safe and effective bridge to transplant in MDS-del5q with transfusion-dependent anemia following lenalidomide relapse: A case report.

Current research in translational medicine·2025
Same author

The use of MSCs in steroid-refractory acute GvHD in Europe: a survey from the EBMT cellular therapy & immunobiology working party.

Bone marrow transplantation·2025
Same author

High-dose chemotherapy with autologous stem cell transplants in adult primary non-seminoma mediastinal germ-cell tumors. A report from the Cellular Therapy and Immunobiology working party of the EBMT.

ESMO open·2024
Same author

"A lactose-modified chitosan accelerates chondrogenic differentiation in mesenchymal stem cells spheroids".

Biomaterials advances·2024

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Regenerative Medicine

Background:

  • Human dental pulp cells possess regenerative potential.
  • Understanding reparative dentinogenesis is crucial for dental tissue engineering.

Purpose of the Study:

  • To simulate reparative dentinogenesis in vitro.
  • To investigate odontoblast differentiation and extracellular matrix deposition.

Main Methods:

  • In vitro culture of human dental pulp cells.
  • Induction of odontogenic differentiation using specific media.
  • Evaluation via Alizarin red staining, transmission electron microscopy, biochemical assays, Real Time PCR, and immunofluorescence.

Main Results:

  • Successful differentiation of dental pulp cells into odontoblast-like cells.

More Related Videos

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy
11:02

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Published on: April 13, 2022

Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

Related Experiment Videos

Last Updated: May 7, 2026

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
07:07

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Published on: January 12, 2017

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy
11:02

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Published on: April 13, 2022

Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

  • Demonstrated mineral deposition and extracellular matrix synthesis (type I collagen).
  • Confirmed expression and localization of key odontoblast markers: Dentin Matrix Protein 1 (DMP1) and Dentin Sialoprotein (DSP).

    • Conclusions:

    • Established an in vitro model for studying reparative dentinogenesis.
    • Provided a comprehensive analysis of cellular and molecular events during odontoblast differentiation.
    • Highlighted the potential for investigating dentin repair mechanisms and mineralization processes.