Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein export in Escherichia coli.

J M Pages, J Anba, C Lazdunski

    Annales De L'Institut Pasteur. Microbiologie
    |January 1, 1985
    PubMed
    Summary

    Hyperproducing the phosphate-binding protein (PhoS) caused export site saturation, accumulating precursor PhoS. Only membrane-associated precursors could be exported, suggesting a translation stop linked to synthesis and export coupling.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cross-resistance between biocides and antimicrobials: an emerging question.

    Revue scientifique et technique (International Office of Epizootics)·2012
    Same author

    Physiological characterisation of the efflux pump system of antibiotic-susceptible and multidrug-resistant Enterobacter aerogenes.

    International journal of antimicrobial agents·2010
    Same author

    An AcrAB-mediated multidrug-resistant phenotype is maintained following restoration of wild-type activities by efflux pump genes and their regulators.

    International journal of antimicrobial agents·2009
    Same author

    Enterobacter gergoviae and the prevalence of efflux in parabens resistance.

    The Journal of antimicrobial chemotherapy·2006
    Same author

    Differential activities of four Lactobacillus casei promoters during bacterial transit through the gastrointestinal tracts of human-microbiota-associated mice.

    Applied and environmental microbiology·2005
    Same author

    Imipenem resistance in Salmonella enterica serovar Wien related to porin loss and CMY-4 beta-lactamase production.

    Antimicrobial agents and chemotherapy·2003

    Area of Science:

    • Molecular Biology
    • Protein Export Mechanisms
    • Cellular Physiology

    Background:

    • Understanding protein export is crucial for cellular function and biotechnological applications.
    • Phosphate-binding protein (PhoS) is essential for phosphate uptake in many organisms.
    • Hyperproduction of secreted proteins can lead to cellular stress and export pathway overload.

    Purpose of the Study:

    • To investigate the consequences of hyperproducing the phosphate-binding protein (PhoS).
    • To elucidate the mechanism of pre-PhoS accumulation and export.
    • To explore the potential existence of a translation stop site involved in PhoS export.

    Main Methods:

    • Analysis of pre-PhoS localization (cytoplasmic vs. membrane-associated) under hyperproduction conditions.
    • Investigating the post-translational maturation and export capabilities of different pre-PhoS pools.
    • Preliminary investigation into mRNA translation dynamics and potential pause sites.

    Main Results:

    • Hyperproduction of PhoS led to saturation of export sites, causing pre-PhoS accumulation in both the cytoplasm and inner membrane.
    • Cytoplasmic pre-PhoS was not exported post-translationally; only membrane-associated precursor could be matured and exported.
    • Evidence suggests a translation stop site in pre-PhoS mRNA occurs after 70-80 amino acids, linked to synthesis-export coupling.

    Conclusions:

    • PhoS hyperproduction disrupts normal export pathways, leading to precursor accumulation.
    • Post-translational export of PhoS is dependent on its association with the membrane.
    • A co-translational mechanism involving a translation pause site appears critical for PhoS export.

    Related Experiment Videos