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Related Concept Videos

IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
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Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...

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Related Experiment Video

Updated: May 7, 2026

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

High-throughput analyses of IP3 receptor behavior.

Colin W Taylor1, Stephen C Tovey, Ana M Rossi

  • 1Department of Pharmacology, University of Cambridge, Cambridge CB2 1PD, United Kingdom.

Cold Spring Harbor Protocols
|October 3, 2013
PubMed
Summary

Inositol 1,4,5-trisphosphate receptors (IP3Rs) are crucial intracellular Ca2+ channels. Understanding how IP3 and Ca2+ regulate IP3R gating is key to deciphering cellular signaling processes.

Area of Science:

  • Cellular Biology
  • Molecular Physiology
  • Biophysics

Background:

  • Inositol 1,4,5-trisphosphate receptors (IP3Rs) are intracellular Ca2+ channels critical for cellular signaling.
  • They are ubiquitously expressed in animal cells, mediating Ca2+ release from the endoplasmic reticulum (ER).
  • IP3Rs respond to stimuli that generate inositol 1,4,5-trisphosphate (IP3), a key second messenger.

Purpose of the Study:

  • To elucidate the complex interplay between IP3 and Ca2+ in controlling IP3R gating.
  • To understand how individual IP3R openings contribute to fundamental Ca2+ signaling events.
  • To explore mechanisms by which Ca2+ signals are amplified through IP3R recruitment.

Main Methods:

  • The study introduces high-throughput methods for investigating IP3R function.

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Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
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Published on: October 15, 2016

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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
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Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
07:26

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

  • Focuses on analyzing the gating of IP3Rs under various conditions.
  • Utilizes techniques to measure Ca2+ dynamics and IP3R activity.

    • Main Results:

    • Individual IP3R openings generate localized, transient Ca2+ signals, acting as fundamental units of Ca2+ signaling.
    • Ca2+-dependent recruitment of neighboring IP3Rs amplifies initial small signals into larger cellular responses.
    • The interplay of IP3 and Ca2+ is crucial for precise regulation of IP3R channel activity.

    Conclusions:

    • Precise control of cellular processes relies on understanding the dual regulation of IP3Rs by IP3 and Ca2+.
    • High-throughput methodologies are essential for dissecting the intricate gating mechanisms of IP3Rs.
    • Further research using these methods will advance our knowledge of Ca2+ signaling pathways.