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Related Experiment Video

Updated: May 7, 2026

Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy
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Published on: May 7, 2018

Motion compensation using a suctioning stabilizer for intravital microscopy.

Claudio Vinegoni, Sungon Lee, Rostic Gorbatov

    Intravital
    |October 3, 2013
    PubMed
    Summary
    This summary is machine-generated.

    A novel suctioning stabilizer minimizes motion artifacts for high-resolution single-cell imaging in organs like the beating heart. This technique enables clear visualization without disrupting physiological functions.

    Keywords:
    cardiac imagingin vivo imagingintravital microscopymotion compensationoptical microscopy

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    Published on: September 30, 2021

    Area of Science:

    • Biomedical Engineering
    • Microscopy
    • Cardiovascular Research

    Background:

    • Motion artifacts significantly impede high-resolution single-cell imaging in dynamic cardiothoracic organs.
    • Existing methods struggle to overcome the challenges posed by physiological movements in real-time imaging.

    Purpose of the Study:

    • To introduce a novel water-immersion suctioning stabilizer for minimally invasive intravital fluorescence microscopy.
    • To demonstrate the stabilizer's efficacy in mitigating motion artifacts in beating organs.

    Main Methods:

    • Development of a water-immersion suctioning stabilizer compatible with water-based stick objectives.
    • Integration of the stabilizer with prospective or retrospective gating for motion correction.
    • Application of the technique to intravital fluorescence microscopy of the beating murine heart.

    Main Results:

    • The stabilizer effectively reduced major motion excursions, enabling cellular resolution imaging.
    • The technique achieved high-resolution imaging in the beating murine heart without perturbing normal physiology.
    • The method allows for probing multiple areas, facilitating wide-area coverage at high resolution.

    Conclusions:

    • The water-immersion suctioning stabilizer is a promising tool for overcoming motion artifacts in cardiothoracic organ imaging.
    • This minimally invasive approach enhances the capability of intravital fluorescence microscopy for physiological studies.
    • The technique offers a pathway for improved in vivo cellular and tissue analysis in dynamic biological systems.