Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Clinical validation of a Novel Robotic Device for MR-Guided Prostate Biopsy: Initial Patient Experience.

Journal of medical robotics research·2026

Same author

Prospective operational feasibility, safety, and workflow of magnetic resonance-guided tracking technology for interstitial gynecologic brachytherapy.

Brachytherapy·2026

Same author

AI-assisted cryo-dose estimation and tissue injury modeling for assessing side effects in MRI-guided prostate cryoablation: a retrospective study.

Physics in medicine and biology·2026

Same author

Machine learning model for fast prediction and uncertainty quantification of needle deflection during prostate biopsy.

Medical physics·2026

Same author

Analysis of Needle Placement Error During Prostate Biopsies in Real Clinical Cases: Effects of Needle Tip Orientation and Target Location.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025

Same author

SlicerROS2: A research and development module for image-guided robotic interventions.

IEEE transactions on medical robotics and bionics·2025

Search research articles

Related Experiment Video

Updated: May 7, 2026

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Published on: March 29, 2024

[Polyethylene glycol accelerates loop-mediated isothermal amplification (LAMP) reaction].

Kenta Nose¹, Kentaro Nagamine, Junichi Tokuda

¹Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Hiroshima International University.

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan

|October 4, 2013

Summary

This study optimized loop-mediated isothermal amplification (LAMP) by adding thickeners, significantly reducing reaction times to under 20 minutes. This faster, cost-effective molecular diagnostic method is ideal for point-of-care testing.

More Related Videos

Detecting SARS-CoV-2 Virus by Reverse Transcription-Loop-Mediated Isothermal Amplification

Detecting SARS-CoV-2 Virus by Reverse Transcription-Loop-Mediated Isothermal Amplification

Published on: September 8, 2023

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Published on: June 25, 2020

Related Experiment Videos

Last Updated: May 7, 2026

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Published on: March 29, 2024

Detecting SARS-CoV-2 Virus by Reverse Transcription-Loop-Mediated Isothermal Amplification

Detecting SARS-CoV-2 Virus by Reverse Transcription-Loop-Mediated Isothermal Amplification

Published on: September 8, 2023

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Published on: June 25, 2020

Area of Science:

Molecular Biology
Biotechnology
Diagnostics

Background:

Loop-mediated isothermal amplification (LAMP) offers advantages over PCR, including isothermal conditions, high specificity, and direct turbidity-based results.
Traditional LAMP is efficient but can be time-consuming compared to the desired speed for rapid diagnostics.

Purpose of the Study:

To reduce the reaction time of the LAMP technique.
To enhance the efficiency and applicability of LAMP for point-of-care testing.

Main Methods:

Modification of LAMP reaction solutions by adding thickeners, specifically polyethylene glycol (PEG) 8000 or 20000.
Monitoring reaction progress via turbidity and fluorescence detection.

Main Results:

Addition of thickeners significantly decreased LAMP reaction time to under 20 minutes.
The modified LAMP method maintained high efficiency and allowed for both turbidity and fluorescence detection.
The enhanced LAMP method proved faster and comparable in cost-effectiveness to traditional PCR.

Conclusions:

Supplementing LAMP reactions with thickeners provides a rapid molecular diagnostic tool.
This accelerated LAMP method is suitable for diverse applications, including hereditary disease diagnosis and viral infection identification at the point-of-care.