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Updated: May 7, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
Routine micromethod for the determination of vitamin K1 in human plasma
Ingolf Meineke1, Cornelia Willnow, Julia Stingl
1*Department of Clinical Pharmacology, University Medical Centre of the Georg August University Göttingen, Göttingen, Germany; and †Institute of Pharmacology of Natural Products and Clinical Pharmacology, University of Ulm, Ulm, Germany.
Background:
A selective and sensitive high-performance liquid chromatographic method with fluorescence detection after postcolumn reduction is described for the routine measurement of vitamin K1 in plasma samples of 100 µL of volume.
Methods:
Liquid-liquid extraction was used for the sample preparation with vitamin K2 as an internal standard. For the chromatographic separation, a standard C18 column was applied. The calibration range used was from 2 to 500 ng/mL.
Results:
At the low level (5 ng/mL), a coefficient of variation of 14.9% was observed interday in the quality control samples, whereas at the intermediate (50 ng/mL) and high (200 ng/mL) levels such of 6.8% and 8.7% were found.
Conclusions:
The method has proven robust and convenient for the analysis of samples from clinical pharmacological studies.
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