Related Experiment Video
Updated: May 7, 2026
Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
Published on: November 10, 2009
Fast protocol for extraction of DNA from Prosopis spp leaves (plant adapted to arid environment) without liquid
C Y Michel-López1, D González-Mendoza, O Grimaldo-Juarez
1Instituto de Ciencias Agrícolas, Universidad Autónoma de Baja California, Baja California, México.
Abstract:
The extraction of high-quality genomic DNA from Prosopis spp for polymerase chain reaction (PCR) amplification is complicated, owing to the presence of a high percentage of secondary metabolites that bind to or co-precipitate with nucleic acids. In the present study, we report a modified sodium dodecyl sulfate/phenol protocol that eliminates the use of liquid nitrogen in the maceration process, β-mercaptoethanol in the buffer extraction, and the ethanol precipitation step. The A₂₆₀/A₂₈₀ absorbance ratios of the isolated DNA were approximately 2.0 to 1.9, suggesting that the DNA fraction was pure and can be used for further PCR analysis. The DNA isolated by this protocol is of sufficient quality for molecular applications; this technique could be applied to other organisms that have similar substances that hinder DNA extraction. Finally, this proposal represents an alternative fast, cheap, and effective method for the isolation of genomic DNA from fresh leaves of Prosopis spp, even in low-technology laboratories.
Related Concept Videos
01:24DNA Isolation
01:34DNA Isolation
02:35DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...