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A new thymocyte membrane differentiation antigen for mouse and rat
Immunogenetics
|January 1, 1985
Summary
Researchers identified a novel thymocyte membrane antigen in mice and rats, distinct from Thy-1. Its expression level on thymocytes is genetically linked to Thy-1, revealing a new antigenic system.
Area of Science:
- Immunology
- Cell Biology
- Genetics
Background:
- A novel thymocyte membrane antigen has been identified in mice and rats.
- This antigen, recognized by monoclonal antibody NIM-M129, shares a similar molecular size with Thy-1 but exhibits a distinct tissue distribution.
Purpose of the Study:
- To define a new thymocyte membrane-brain antigenic system in mice and rats.
- To investigate the genetic linkage and expression patterns of this novel antigen in relation to Thy-1.
Main Methods:
- Monoclonal antibody characterization (NIM-M129).
- Analysis of antigen expression in various thymocyte populations and tissues.
- Genetic segregation tests (backcross and F2) using congenic mouse lines.
Main Results:
- The antigen is absent in cortisone-resistant thymocytes, peripheral T cells, T-cell blasts, lymphomas, and rat bone marrow.
- Quantitative differences in expression were observed between Thy-1a (low) and Thy-1b (high) mice, with intermediate levels in F1 progeny.
- Genetic linkage was established between Thy-1 and the level of epitope expression on thymocytes.
Conclusions:
- A new thymocyte membrane-brain antigenic system has been defined.
- The expression of this novel antigen on thymocytes is genetically controlled and linked to the Thy-1 locus.