Related Experiment Video
Updated: May 7, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Snapshot of a DNA polymerase while incorporating two consecutive C5-modified nucleotides
Samra Obeid1, Holger Bußkamp, Wolfram Welte
1Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, University of Konstanz , Universitätsstrasse 10, 78457 Konstanz, Germany.
Abstract:
Functional nucleotides are important in many cutting-edge biomolecular techniques. Often several modified nucleotides have to be incorporated consecutively. This structural study of KlenTaq DNA polymerase, a truncated form of Thermus aquaticus DNA polymerase, gives first insights how multiple modifications are processed by a DNA polymerase and, therefore, contribute to the understanding of these enzymes in their interplay with artificial substrates.
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