Demonstration of allospecific differences in human stratum corneum autoantibodies by blocking immunofluorescence

Stratum corneum antibody titers of six normal human sera were determined by indirect immunofluorescence on three different epidermal substrates with and without preincubation of the substrates with one monkey serum. In three out of six human sera the reaction of stratum corneum antibodies was inhibited on all three substrates, in one serum the inhibition was noted on two out of three substrates and no effect was observed in two sera. These findings provide further evidence for the existence of allospecific stratum corneum antibodies in human sera and for the presence of both species-specific and species-nonspecific glycoprotein stratum corneum antigens in human epidermis.