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Related Experiment Video

Updated: May 7, 2026

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)
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A WAO - ARIA - GA²LEN consensus document on molecular-based allergy diagnostics.

Giorgio Walter Canonica1, Ignacio J Ansotegui, Ruby Pawankar

  • 1Allergy & Respiratory, DIMI, Department of Internal Medicine, University of Genoa, Largo Rosanna Benzi, Genoa, Italy. canonica@unige.it.

The World Allergy Organization Journal
|October 5, 2013
PubMed
Summary

Molecular-based allergy (MA) diagnostics precisely maps allergen sensitization using specific molecules. This approach enhances allergy diagnosis accuracy, identifies triggers for immunotherapy, and predicts reaction severity.

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Area of Science:

  • Allergy Diagnostics
  • Immunology
  • Molecular Biology

Background:

  • Molecular-based allergy (MA) diagnostics analyzes allergen sensitization at a molecular level using purified allergenic molecules.
  • Over 130 allergenic molecules are available for in vitro specific IgE (sIgE) testing, integrating MA diagnostics into routine care.

Purpose of the Study:

  • To provide a practical guide for clinicians on the indications, determination, and interpretation of MA diagnostics.
  • To update allergists on the rapidly expanding evidence base for MA diagnosis.

Main Methods:

  • Utilizes purified natural or recombinant allergenic molecules (allergen components) for in vitro specific IgE (sIgE) testing.
  • Employs singleplex and multiplex measurement platforms, including the comprehensive Immuno-Solid phase Allergen Chip (ISAC) biochip technology.

Main Results:

  • MA diagnostics improves allergy diagnosis accuracy and prognosis.
  • It differentiates genuine from cross-reactive sensitization, assesses food allergy reaction severity, and identifies candidates for specific immunotherapy (SIT).

Conclusions:

  • MA diagnostics offers increased accuracy in allergy diagnosis and prognosis.
  • Future research should focus on large-scale studies, expanding molecular targets, and supporting test interpretation.
  • Clinicians require updated information to effectively utilize MA diagnostics.