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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
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Single amino acid substitutions in recombinant plant-derived human α1-proteinase inhibitor confer enhanced stability

Shweta Jha1, Indraneel Sanyal, D V Amla

  • 1Plant Transgenic Lab, MB and GE Division, CSIR-National Botanical Research Institute, P.O. Box 436, Rana Pratap Marg, Lucknow 226 001, India.

Biochimica Et Biophysica Acta
|October 5, 2013
PubMed

Keywords:
C(m)CDDAC-ELISAHuman α(1)-proteinase inhibitorOxidation resistancePPEProtein stabilityRCLRecombinant therapeutic proteinSingle amino acid substitutionTSPTUGTransgenic plantcircular dichroismdirect antigen coating-enzyme linked immunosorbent assaymid-point of transitionporcine pancreatic elastasereactive center looptotal soluble proteintransverse urea gradient gel electrophoresisα(1)-PIα(1)-proteinase inhibitor

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