Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

6.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.1K
Lineage Commitment01:21

Lineage Commitment

3.4K
Commitment is the  process whereby stem cells:
3.4K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.1K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.1K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.4K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.4K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

10.2K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
10.2K
T Cell Types and Functions01:24

T Cell Types and Functions

3.2K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-compartment immune and tumor cell reprogramming by IFNα2 overcomes colon cancer immunotherapy resistance.

bioRxiv : the preprint server for biology·2026
Same author

B cell-intrinsic IRF8 transcriptionally reprograms antigen presentation to sustain CD8⁺ T cell antitumor immunity.

bioRxiv : the preprint server for biology·2026
Same author

H3K9me3 regulates IFNγlo Tex cell differentiation to promote colon tumor liver metastasis.

Molecular cancer therapeutics·2026
Same author

Deep Learning-Based Structure Modeling of the <i>Treponema pallidum</i> Proteome: Insights into Pathogenesis and Syphilis Vaccine Development.

bioRxiv : the preprint server for biology·2026
Same author

Improving Access to Community Occupations via Rideshare: A Pilot Study With Autistic Adults.

The American journal of occupational therapy : official publication of the American Occupational Therapy Association·2026
Same author

Distinct and cooperative roles of host and tumor Osteopontin in colorectal cancer liver metastasis.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: May 5, 2026

Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS
14:15

Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS

Published on: June 18, 2012

25.7K

Myeloid-derived suppressor cell development is regulated by a STAT/IRF-8 axis.

Jeremy D Waight, Colleen Netherby, Mary L Hensen

    The Journal of Clinical Investigation
    |October 5, 2013
    PubMed
    Summary

    Interferon regulatory factor-8 (IRF-8) deficiency drives the development of myeloid-derived suppressor cells (MDSCs). Restoring IRF-8 levels may offer new strategies to target MDSCs in cancer therapy.

    More Related Videos

    Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
    10:21

    Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

    Published on: February 21, 2018

    9.3K
    Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
    06:12

    Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

    Published on: March 7, 2022

    3.0K

    Related Experiment Videos

    Last Updated: May 5, 2026

    Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS
    14:15

    Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS

    Published on: June 18, 2012

    25.7K
    Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
    10:21

    Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

    Published on: February 21, 2018

    9.3K
    Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
    06:12

    Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

    Published on: March 7, 2022

    3.0K

    Area of Science:

    • Immunology
    • Cancer Biology
    • Molecular Biology

    Background:

    • Myeloid-derived suppressor cells (MDSCs) are immature myeloid cells that impair anti-tumor immunity and pose a barrier to cancer therapy.
    • The precise mechanisms governing MDSC development and their granulocytic bias remain incompletely understood.
    • Aberrant myelopoiesis, potentially linked to transcriptional regulators, is implicated in MDSC generation.

    Purpose of the Study:

    • To investigate the role of interferon regulatory factor-8 (IRF-8) in the development and regulation of MDSCs.
    • To determine if IRF-8 perturbations contribute to the aberrant myelopoiesis observed in MDSC populations.
    • To explore the therapeutic potential of modulating IRF-8 levels for cancer treatment.

    Main Methods:

    • Utilized Irf8-deficient mice to model MDSC development.
    • Overexpressed IRF-8 in mice to assess its impact on MDSC accumulation and immunotherapy.
    • Investigated the influence of G-CSF and GM-CSF on IRF-8 expression via STAT3 and STAT5 pathways.
    • Analyzed IRF-8 levels in MDSCs from breast cancer patients.

    Main Results:

    • Irf8-deficient mice exhibited myeloid populations phenotypically and functionally similar to tumor-induced MDSCs.
    • IRF-8 overexpression reduced MDSC accumulation and improved the efficacy of cancer immunotherapy.
    • Granulocyte-colony stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) downregulated IRF-8 through STAT3/STAT5.
    • Decreased IRF-8 levels correlated with increased MDSC frequency in breast cancer patients.

    Conclusions:

    • IRF-8 acts as a critical negative regulator in the development of myeloid-derived suppressor cells.
    • Dysregulation of IRF-8 contributes to the expansion of MDSCs in neoplastic conditions.
    • Targeting IRF-8 presents a novel therapeutic strategy for overcoming MDSC-mediated immunosuppression in cancer.