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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Large-scale study of long non-coding RNA functions based on structure and expression features.

Yi Zhao1, Jian Wang, Xiaowei Chen

  • 1Bioinformatics Research Group, Advanced Computing Research Laboratory, Institute of Computing Technology, Chinese Academy of Sciences, Beijing, 100190, China.

Science China. Life Sciences
|October 5, 2013
PubMed
Summary

This review explores methods for analyzing long non-coding RNA (lncRNA) structure and expression. Understanding lncRNA characteristics is crucial for deciphering their regulatory roles in complex biological processes.

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Published on: July 22, 2016

Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Complex organisms, including mammals, transcribe numerous long non-coding RNAs (lncRNAs).
  • lncRNAs play diverse regulatory roles in biological processes.
  • lncRNA functions are largely determined by their structure and expression levels.

Purpose of the Study:

  • To review current methodologies for analyzing lncRNA structure.
  • To review current methodologies for analyzing lncRNA expression.
  • To provide foundational information for large-scale lncRNA functional studies.

Main Methods:

  • Literature review of established and emerging techniques.
  • Analysis of methods for structural elucidation of lncRNAs.
  • Evaluation of techniques for quantifying lncRNA expression.

Main Results:

  • A comprehensive overview of current analytical methods for lncRNA structure and expression is presented.
  • Key techniques and their applications in lncRNA research are discussed.
  • The importance of accurate structural and expression data is highlighted.

Conclusions:

  • Accurate analysis of lncRNA structure and expression is fundamental for understanding their biological functions.
  • This review serves as a resource for researchers investigating lncRNA roles.
  • Further development of analytical methods will enhance large-scale functional genomics studies.