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Updated: Aug 10, 2026

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses
Published on: February 18, 2016
Triple immunological staining with colloidal gold, fluorescein and rhodamine as labels
Abstract:
Colloidal gold particles are detectable by light microscopy with polarized light in the same epi-illumination system as for fluorescence microscopy. Colloidal gold particles can thus be used in combination with a fluorochrome for the combined immunological detection of surface membrane and cytoplasmic markers. We analyzed human bone marrow cells by a triple immunological staining for 3 different markers, using colloidal gold, fluorescein and rhodamine as labels. Our results demonstrate that such a triple immunological staining provides a powerful tool for study of the heterogeneity of small cell populations.
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